Quantifying anti-HBV effect of targeted ribonuclease by real-time fluorescent PCR

• 作 者: Jun Liu Ying-Hui Li Jin Ding Wei-Dong Gong Cai-Fang Xue Ya Zhao Yu-Xiao Huang
• 机 构: Department of Etiology,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China
• 出 处: 世界胃肠病学杂志（英文版）
• 发表日期: 2004年19期
• 关键词: HBV replication HBV DNA

AIM: To quantify the inhibition of HBV replication by targeted ribonuclease by using real-time fluorescent PCR.METHODS: Targeted ribonuclease was introduced into 2.2.15cells by liposome-mediated transfection or HIV-TAT mediated protein transduction. Forty-eight hours after the transfection and 24 h after the transduction, supatants of 2.2.15 cells were collected and HBV DNA in the supatants was quantified by real-time fluorescent PCR with a commercial kit.RESULTS: HBV DNA concentrations in the supatants of2.2.15 cells transfected or transducted with targeted ribonuclease were 4.9±2.4×108 copies/L and 8.3±4.0×108copies/L, respectively. Compared with controls, transfection or transduction of targeted ribonuclease reduced HBV DNA concentration in the supatants of 2.2.15 cells by 90.4%and 90.1%, respectively (P＜0.05).CONCLUSION: Targeted ribonuclease can inhibit HBV replication in 2.2.15 cells.