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目的探讨S期激酶相关蛋白2(Skp2)及磷酸酶和张力蛋白基因(PTEN)在不同子宫内膜组织中的表达及其与子宫内膜癌发生、发展的关系。方法选取中国医科大学附属盛京医院2008-2010年手术切除的59例子宫内膜腺癌标本。患者年龄36～70岁,平均54岁。另选取30例不典型增生子宫内膜和30例增生期子宫内膜标本,应用免疫组织化学SP法和免疫印迹法(western blotting)检测Skp2和PTEN蛋白的表达水平,分析其表达水平与临床病理参数的关系以及二者的相互关系。结果 Skp2在子宫内膜癌中表达高于不典型增生和正常增生期内膜,差异有统计学意义(P<0.05),在晚期子宫内膜癌、低分化组和有淋巴结转移组较早期、高中分化组和无淋巴结转移组表达增高,差异有统计学意义(P<0.05)。PTEN在子宫内膜癌中的表达低于不典型增生和正常增生期内膜,差异有统计学意义(P<0.05),在晚期子宫内膜癌和低分化组表达低于早期和高中分化组,差异有统计学意义(P<0.05)。在子宫内膜癌中Skp2表达增高,PTEN表达下降,二者呈负相关。结论 Skp2和PTEN可能参与子宫内膜癌的发生发展过程。
Objective To investigate the expression of Skp2, phosphatase and tensin gene (PTEN) in different endometrial tissues and their relationship with the occurrence and development of endometrial carcinoma. Methods Fifty-nine cases of endometrial adenocarcinoma surgically resected from Shengjing Hospital Affiliated to China Medical University from 2008 to 2010 were selected. Patients aged 36 to 70 years, mean 54 years. Another 30 cases of atypical hyperplasia endometrium and 30 cases of endometrial hyperplasia specimens were selected immunohistochemical SP method and Western blotting (Western blotting) Skp2 and PTEN protein expression levels were analyzed, the expression level and clinical pathology The relationship between the parameters and the relationship between the two. Results The expression of Skp2 in endometrial carcinoma was higher than that in atypical hyperplasia and normal hyperplasia (P <0.05). In early stage of endometrial carcinoma, poorly differentiated group and lymph node metastasis group, High school differentiation group and no lymph node metastasis group increased expression, the difference was statistically significant (P <0.05). The expression of PTEN in endometrial carcinoma was lower than that in atypical hyperplasia and normal hyperplasia (P <0.05), and it was lower in advanced endometrial carcinoma and poorly differentiated group than in early and high differentiated group , The difference was statistically significant (P <0.05). In endometrial carcinoma Skp2 expression increased, PTEN expression decreased, the two was negatively correlated. Conclusion Skp2 and PTEN may participate in the development of endometrial cancer.