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目的和方法 :选用C5 7BL种系环加氧酶 2 (cyclooxygenase 2 ,COX 2 )缺陷小鼠 ,腹腔注射 1 甲基 4 苯基 1,2 ,3,6 四氢吡啶 (MPTP)制备帕金森病小鼠模型 ,用免疫组织化学方法观察COX 2对帕金森病小鼠黑质多巴胺能神经元的影响。结果 :行为学及免疫组织化学观察显示 ,野生型帕金森病小鼠的死亡率明显高于COX 2缺陷杂合子帕金森病小鼠 (P <0 .0 1) ,野生型帕金森病小鼠黑质致密部酪氨酸羟化酶 (tyrosinehydroxylase,TH)免疫反应阳性神经元数目较杂合子帕金森病小鼠明显减少 (P <0 .0 1)。结论 :COX 2可能与帕金森病时黑质多巴胺能神经元的损伤有关
PURPOSE AND METHODS: Parkinson’s disease was induced by intraperitoneal injection of 1-methyl-4-phenyl 1,2,6,6-tetrahydropyridine (MPTP) in C5 7BL-deficient mice with cyclooxygenase 2 (COX 2) Mouse model, the effect of COX 2 on nigral dopaminergic neurons in Parkinson’s disease mice was observed by immunohistochemistry. Results: Behavioral and immunohistochemical studies showed that the mortality of wild-type mice with Parkinson’s disease was significantly higher than that of mice with heterozygous COX 2-deficient Parkinson’s disease (P <0.01), wild-type Parkinson’s disease The number of tyrosine hydroxylase (TH) immunoreactive neurons in the substantia nigra pars compacta was significantly lower than that in heterozygous Parkinson’s disease mice (P <0.01). Conclusion: COX 2 may be related to the damage of substantia nigra dopaminergic neurons in Parkinson’s disease