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目的 :探讨脑缺血及再灌流内皮素、降钙素基因相关肽和神经元凋亡变化规律及关系。方法 :前脑缺血及再灌流大鼠为模型 ,检测海马ET、CGRP含量和神经元凋亡。结果 :缺血后 1 5minET显著高于其他各组 ;再灌流后降低 ,1d时最低 ,2d时恢复正常。缺血后 1 5minCGRP显著低于其他各组 ;再灌流后升高 ,1d达高峰后下降。缺血后出现神经元凋亡 ,再灌流后仍增加 ,1d达高峰后下降。ET含量与凋亡细胞呈负相关。CGRP含量与凋亡细胞呈正相关。结论 :缺血和再灌流均可导致神经元凋亡。
Objective: To investigate the regularity of the changes of apoptosis of endothelin, calcitonin gene related peptide and neurons in cerebral ischemia and reperfusion. Methods: The models of forebrain ischemia and reperfusion were used to detect the content of ET and CGRP and the apoptosis of neurons in the hippocampus. Results: The ischemic post-ischemic time was significantly higher than that of the other groups at 15 min after ischemia; after reperfusion, it was lower at 1 d and returned to normal at 2 d. At 15 min after ischemia, the level of CGRP was significantly lower than that of other groups. After reperfusion, the level of CGRP increased and reached a peak at 1 d, then decreased. Neuronal apoptosis occurred after ischemia, increased after reperfusion, and reached the peak on the 1st day and then decreased. ET content and apoptotic cells was negatively correlated. CGRP content was positively correlated with apoptotic cells. Conclusion: Both ischemia and reperfusion can lead to neuronal apoptosis.