【摘 要】
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Despite the wide application of super-resolution (SR) microscopy in biological studies of cells,the technology is rarely used to monitor functional changes in l
【机 构】
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State Key Laboratory of Membrane Biology,Beijing Key Laboratory of Cardiometabolic Molecular Medicin
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Despite the wide application of super-resolution (SR) microscopy in biological studies of cells,the technology is rarely used to monitor functional changes in live cells.By combining fast spinning disc-confocal structured illumination microscopy (SD-SIM)with loading of cytosolic fluorescent Ca2+ indicators,we have developed an SR method for visualization of regional Ca2+dynamics and related cellular organelle morphology and dynamics,termed SR calcium lantern imaging.In COS-7 cells stimulated with ATP,we have identified various calcium macrodomains characterized by different types of Ca2+ release from endoplasmic reticulum (ER) stores.Finally,we demonstrated various roles of mitochondria in mediating calcium signals from different sources;while mitochondria can globally potentiate the Ca2+ entry associated with store release,mitochondria also locally control Ca2+ release from the neighboring ER stores and assist in their refilling processes.
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