用双特异性抗体连接人外周血淋巴细胞释放抑制肿瘤生长的细胞因子

• 作 者: 何金妹 余琪
• 出 处: 国外医学(免疫学分册)
• 发表日期: 1991年06期
• 关键词: 抗肿瘤抗体 双特异性抗体 效应细胞 肿瘤 细胞因子 细胞生长抑制率 肿瘤抗体 肿瘤生长抑制 细胞亚群 肿瘤细胞株

作者从正常供者的外周血中分离淋巴细胞作为效应细胞,在96孔板上进行肿瘤生长抑制实验。先将能结合效应细胞亚群的抗体(抗CD_3、抗CD_4,抗CD_8等)分别加入孔中,这些抗体可预先交联或未交联特异性抗肿瘤抗体;然后加入效应细胞或其培养上清液;最后加入人的各种肿瘤细胞株。用MTT标记法计算肿瘤细胞生长抑制率。

The authors isolated lymphocytes from the peripheral blood of normal donors as effector cells and performed tumor growth inhibition experiments on 96-well plates. Antibodies (anti-CD_3, anti-CD_4, anti-CD_8, etc.) capable of binding to effector cell subsets are added to wells, respectively, and these antibodies can be pre-crosslinked or non-crosslinked with specific antitumor antibodies; then, effector cells or their culture Serum; the last to join the various human tumor cell lines. Tumor cell growth inhibition rate was calculated by MTT labeling method.