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目的观察2例慢性HCV携带者及1例HCVRNA转阴者外周血淋巴细胞对HCVNS3区辅助性T细胞抗原表位的应答。方法用血清学方法检测患者的HLA分型。根据计算机软件分析和文献资料 ,合成了1个位于HCVNS3区长度为14个氨基酸的辅助性T细胞表位 ,与在GeneBank中已发表的34个HCV全长序列进行了比较。用T细胞增殖实验方法检测对这一表位的应答。结果3例患者的MHCII类分子不完全相同。这一表位在已发表的34个HCV全长序列中高度保守 ,只在4个序列有1个氨基酸的差异。本实验发现 ,无论是两例长期HCV患者 ,还是1例HCVRNA转阴者 ,对此多肽的刺激均有较强的应答 ,刺激指数均大于3 ,而8例正常对照组均小于1.6。结论对HCVNS3区的这一辅助性T细胞表位的应答强度 ,不仅与HCV的自限性有关 ,而且与慢性HCV感染的发病状况有关。
Objective To observe the response of peripheral blood lymphocytes from 2 chronic HCV carriers and 1 HCV HCV negative patients to the epitopes of HCVNS3 helper T cells. Methods Serological methods were used to detect HLA typing. Based on computer software analysis and literature data, a 14-amino acid helper T cell epitope in HCV NS3 region was synthesized and compared with 34 published full-length HCV sequences in GeneBank. T cell proliferation assay was used to test for response to this epitope. Results The MHC class II molecules in 3 patients were not exactly the same. This epitope is highly conserved among the 34 published full-length HCV sequences with only 1 amino acid difference in the 4 sequences. The experiment found that, whether it is two cases of long-term HCV patients, or one case of HCV RNA negative people, the peptides have a strong response to stimulation, stimulation index was greater than 3, while 8 normal control group were less than 1.6. Conclusion The strength of response to this helper T cell epitope in HCV NS3 region is not only related to the self-limiting nature of HCV but also to the pathogenesis of chronic HCV infection.