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目的建立测定安胃片中的延胡索乙素的含量的方法。方法色谱柱为sunfire C18,流动相为乙腈-水(40:60,磷酸调pH6.4),检测波长为283nm,流速为1.0ml·min-1,柱温25℃。结果延胡索乙素浓度3.0～112.5μg·ml-1与峰面积的线性关系良好(r=0.9999),平均加样回收率为98.56%,RSD为2.32%。结论所建方法准确、重复性好,可用于测定安胃片中的延胡索乙素,以确保产品的质量和临床疗效。
Objective To establish a method for the determination of tetrahydropalmatine in Anwei Tablets. Method The column was sunfire C18, the mobile phase was acetonitrile-water (40:60, pH adjusted to pH6.4), the detection wavelength was 283 nm, the flow rate was 1.0 ml·min-1, and the column temperature was 25°C. Results The linear relationship between the concentration of tetrahydropalmatine and the peak area was 3.0～112.5μg·ml-1 (r=0.9999). The average recovery was 98.56% and the RSD was 2.32%. Conclusion The established method is accurate and reproducible, and can be used for the determination of tetrahydropalmatine in Anwei tablets to ensure the quality and clinical efficacy of the product.