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对脱落于精液中的生精细胞,用瑞-吉染色法可使细胞核着色清晰度好;用伊红-瑞-吉染色法能区别死亡和活的精子,并使精子顶体着色清晰;用苯胺黑-伊红-瑞-吉染色法精子核染成红色,精子鞭毛不着色;用煌绿-瑞-吉染色法,成熟精子着色浅,不成熟精子头部着紫红色;过氧化物酶-瑞-吉混合染色,中性白细胞核呈紫红色,胞浆呈浅绿色。5种染色方法中瑞-吉法对生精细胞染色效果最好,可视精液检查的不同要求选用不同方法。
For spermatogenic cells shedding into the semen, the color of the nucleus is well defined by Rui-Kyr staining; the death and viable spermatozoa can be distinguished from the spermatozoa and the spermatozoa can be clearly colored by using the eosin-Rui-Kyr staining method; Aniline black-eosin-Swiss-Kyrgyz staining sperm nuclei were stained red, sperm flagella no staining; with brilliant green - Swiss - Kyrgyzstan staining, mature sperm pale, immature sperm head purple; peroxidase - Swiss - Kyrgyzstan mixed staining, purplish red nucleus was white, pale green cytoplasm. 5 kinds of staining methods Swiss - Kyrgyzstan spermatogenic cells staining the best, visual inspection of the different requirements of different methods used.