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为了建立高效稳定的胰岛素抵抗2型糖尿病(T2DM)模型并观察该模型的稳定性,试验以高脂高糖联合氧化剂叔丁基过氧化氢(t BHP)0.005 m L/g诱导建立胰岛素抵抗T2DM模型,测定SD大鼠不同时间点的体重、空腹血糖(FBG)、三酰甘油(TG)、总胆固醇(CHO)、胰岛素(FINS)、胰岛素敏感指数(ISI),将24 h FBG≥10.0 mmol/L及ISI≤正常动物平均值的大鼠定为T2DM模型大鼠;然后连续3 d给T2DM模型大鼠灌胃盐酸二甲双胍片75 mg/kg,研究该模型的有效性。结果表明:4周后,与对照组、双高组相比,t BHP组大鼠体重、FBG、TG、CHO、FINS、ISI均差异显著(P<0.05);该T2DM模型在4周内非空腹血糖(NFBG)水平较高;盐酸二甲双胍片能降低该T2DM模型的NFBG。说明建立的胰岛素抵抗T2DM模型具有易行、稳定的特点。
To establish an efficient and stable T2DM model of insulin resistance and to observe the stability of the model, insulin resistance T2DM was induced by high-fat and high-glucose (t BHP) 0.005 m L / g The body weight, fasting blood glucose (FBG), triglyceride (TG), total cholesterol (CHO), insulin (FINS) and insulin sensitivity index (ISI) of SD rats at different time points were determined. / L and ISI ≤ normal animals, rats were assigned to T2DM model rats; and then fed with DMEM hydrochloride 75 mg / kg for 3 consecutive days to study the effectiveness of this model. The results showed that body weight, FBG, TG, CHO, FINS and ISI in t BHP group were significantly different from those in control group and double-high group after 4 weeks (P <0.05) Fasting blood glucose (NFBG) levels higher; metformin hydrochloride tablets can reduce NFBG of the T2DM model. The established insulin resistance T2DM model has the characteristics of easy and stable.