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目的探讨孤儿核受体Nur77激动剂CsnB对人HepG2肝癌细胞胆固醇代谢调控基因的影响。方法利用CsnB诱导Nur77在人HepG2肝癌细胞中的高表达。通过与CsnB相同剂量的DMSO对照组的比较,观察CsnB作用不同时间后肝细胞胆固醇代谢相关重要基因的变化,如低密度脂蛋白受体(LDLR)、HMGCoA还原酶(HMGCR)和肝X受体α(LXRα)。结果 10μg/ml终浓度CsnB刺激HepG2细胞可以达到Nur77的高表达,并在作用1.5h后表达量达到高峰。相同浓度CsnB刺激HepG2细胞后,LDLR与HMGCR随时间的延长逐渐下降,并且两种基因在CsnB处理24h后的表达量与0h比较,差异有统计学意义(P<0.05);而LXRα的表达量变化不大。结论 CsnB可以有效诱导Nur77在HepG2细胞中的高表达,其对于肝癌细胞胆固醇代谢调控基因的影响主要表现为LDLR与HMGCR的下调。
Objective To investigate the effects of CsnB, an orphan nuclear receptor, on the cholesterol metabolism regulatory genes in human HepG2 hepatocarcinoma cells. Methods CsnB was used to induce the high expression of Nur77 in human HepG2 hepatoma cells. The effects of CsnB on the changes of hepatic cholesterol metabolism related genes such as LDLR, HMGCoA reductase (HMGCR) and liver X receptor α (LXRα). Results The expression of Nur77 was up-regulated in HepG2 cells stimulated with CsnB at the final concentration of 10μg / ml, and peaked at 1.5h. The same concentration of CsnB stimulated HepG2 cells, LDLR and HMGCR gradually decreased over time, and the two genes in CsnB 24h treatment compared with 0h, the difference was statistically significant (P <0.05), and the expression of LXRα Has not changed much. Conclusion CsnB can effectively induce the high expression of Nur77 in HepG2 cells. The effects of CsnB on the regulation of cholesterol metabolism in hepatocellular carcinoma cells are mainly the down-regulation of LDLR and HMGCR.