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随着拉米夫定应用时间的延长,病毒变异的问题日渐突出,不同肝病患者出现病毒变异后情况不同,因此应给予不同的治疗对策。 1.资料与方法:3例患者均为在我科住院或门诊治疗的乙型肝炎病例。肝功能使用全自动生化分析仪检测,乙型肝炎病毒(HBV)标志物采用微粒子酶免荧光分析法(MEIA)检测,试剂使用美国雅培公司提供的Abbott试剂盒,HBV DNA采用荧光聚合酶链反应(PCR)定量检测,YMDD变异采用限制性内切酶长度多态性(IFLP)分析法检测。病例1,男,41岁。发现HBV感染12年,肝功能异常2年,HBV复制指标阳性。诊断:慢性乙
With the extension of the application of lamivudine, the problem of virus mutation has become increasingly prominent. Different liver disease patients have different situations after virus mutation. Therefore, different treatment strategies should be given. 1. Materials and Methods: All three patients were hepatitis B patients who were hospitalized or outpatient in our department. The liver function was detected by automatic biochemical analyzer. Hepatitis B virus (HBV) markers were detected by microparticle enzyme immunoassay (MEIA). Abbott kit provided by Abbott Laboratories Inc. was used as the reagent. Fluorescent polymerase chain reaction (PCR) quantitative detection, YMDD mutation using restriction endonuclease length polymorphism (IFLP) assay. Case 1, male, 41 years old. HBV infection found 12 years, 2 years of abnormal liver function, HBV replication was positive. Diagnosis: Chronic B