Construction of a Plasmid and a Standard Curve for Real-time Quantitative PCR of the Cold-induced Co

• 出 处: 食用菌学报
• 发表日期: 2007年03期
• 关键词: Plasmid plasmid ultimately DNA template stored reporter agarose contained transf

A plasmid containing target DNA and a standard curve for real-time quantitative PCR of the cold-induced Cor3 gene of Volvariella volvacea were constructed. These will provide the basis for further research on Cor3 gene expression at low temperature, and ultimately for assigning a role for the gene in the low temperature autolysis of V. volvacea.

A plasmid containing target DNA and a standard curve for real-time quantitative PCR of the cold-induced Cor3 gene of Volvariella volvacea were constructed. These will provide the basis for further research on Cor3 gene expression at low temperature, and ultimately for assigning a role for the gene in the low temperature autolysis of V. volvacea.