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目的:本实验旨在通过动物实验观察小肠缺血预适应对急性心梗大鼠抗氧化系统及细胞凋亡的影响。为中医理论“心合小肠”提供现代医学证据。方法:选用体重在160～250g之间的健康雄性SD大鼠120只随机分为5组,分别为小肠缺血预适应2min组,小肠缺血预适应5min组,小肠缺血预适应10min组,小肠假手术+心梗手术组,直接心梗手术组。采用手术的方法进行小肠缺血预适应并用冠状动脉结扎法制造心梗模型。造模成功后取血检测NO。结果:缺血预适应组与小肠假手术+心梗手术组、直接心梗组比较,心梗范围明显减少(P<0.05),血清中NO含量明显增多(P<0.05,P<0.01)。结论:小肠缺血预适应可能通过抵抗氧自由基对心肌细胞生物膜的损伤作用,从而对心肌细胞起到保护作用。
Objective: The purpose of this experiment is to observe the effect of intestinal ischemia preconditioning on the anti-oxidative system and apoptosis of acute myocardial infarction rats through animal experiments. Provide modern medical evidence for TCM theory “heart combined with small intestine”. Methods: One hundred and twenty healthy male Sprague-Dawley rats weighing 160-250g were randomly divided into 5 groups: preconditioning of intestinal ischemia for 2 min, intestinal ischemia preconditioning for 5 min, intestinal ischemia preconditioning for 10 min, Small intestinal pseudo-surgery + myocardial infarction group, direct myocardial infarction group. Surgical methods were used to precondition intestinal ischemia and the myocardial infarction model was made by coronary ligation. After the success of modeling blood test NO. Results: The extent of myocardial infarction in ischemic preconditioning group was significantly lower than that in sham-operated + MI group and direct MI group (P <0.05). The content of NO in serum increased significantly (P <0.05, P <0.01). Conclusions: Intestinal ischemia preconditioning may play a protective role on cardiomyocytes by resisting the injury of oxygen free radicals on the myocardial cells.