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目的:探讨过表达癌胚抗原相关黏附分子6(carcinoembryonic antigen-related cell adhesion molecule 6,CEACAM6)对胃癌细胞凋亡与失巢凋亡的影响。方法:构建CEACAM6真核过表达载体转染胃癌细胞SGC-7901和MKN-45,使其CEACAM6过表达,qRT-PCR、Western印迹验证过表达效果,免疫荧光定位CEACAM6;流式细胞仪检测过表达CEACAM6后胃癌细胞凋亡的变化,失巢凋亡检测其失巢凋亡的变化。结果:转染CEACAM6后,胃癌细胞SGC-7901和MKN-45的CEACAM6分别过表达了30 000倍和4 000倍;在胃癌细胞SGC-7901中凋亡与失巢凋亡分别下降了46.2%和53.8%,在MKN-45中凋亡与失巢凋亡分别下降了71.7%和74.6%。结论:过表达CEACAM6抑制胃癌细胞的凋亡和失巢凋亡,CEACAM6通过抑制胃癌细胞凋亡和失巢凋亡的途径参与胃癌细胞的恶性生长过程。
Objective: To investigate the effect of carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) on apoptosis and anoikis in gastric cancer. Methods: CEACAM6 eukaryotic overexpression vector was constructed and transfected into gastric cancer cell line SGC-7901 and MKN-45 to overexpress CEACAM6. The overexpression of CEACAM6 was confirmed by qRT-PCR and Western blotting. CEACAM6 was detected by immunofluorescence staining. The change of apoptosis of gastric cancer cell line CEACAM6 and the change of anoikis after anoikis were detected. Results: After CEACAM6 transfection, CEACAM6 in SGC-7901 and MKN-45 cells were over-expressed 30 000-fold and 4000-fold, respectively. The apoptosis and anoikis decreased in SGC-7901 cells by 46.2% and 53.8%. The apoptosis and anoikis in MKN-45 decreased by 71.7% and 74.6% respectively. CONCLUSION: CEACAM6 can inhibit the apoptosis and anoikis in gastric cancer cells. CEACAM6 is involved in the malignant growth of gastric cancer cells by inhibiting the apoptosis of gastric cancer cells and anoikis.