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银纳米粒子(AgNPs)是一种潜在的抗菌材料,会引起细胞膜的损伤,并能产生活性氧(ROS)。由此产生的细胞膜的结构性变化能够增加银离子和AgNPs在细胞中的渗透能力。为了进一步阐述这个问题,我们测试了这种纳米材料的体内和体外细胞毒性,并通过这种方法来量化和评估它们的纳米毒性。考虑到AgNPs在环境中的行为,毒性可能受到其物理化学性质(尺寸、附聚率、在腐植酸上的吸附性能)和毒性抑制性质的差异影响。我们通过附聚和吸附动力学研究了细胞吸收AgNPs的作用。试验发现,尺寸小于14nm的AgNPs的附聚和吸附高于尺寸为90nm和140nm的AgNPs的附聚和吸附。对于大小为90nm和140nm的AgNPs,吸附作用显著大于附聚作用。值得注意的是,在正规概念下,AgNPs越小越容易被吸收这一正常概念在非生物因素相互作用下可能是错误的。
Silver nanoparticles (AgNPs) are a potential antimicrobial material that causes cell membrane damage and produces reactive oxygen species (ROS). The resulting structural changes of the cell membrane can increase the penetration of silver ions and AgNPs in the cells. To further elucidate this issue, we tested the in vivo and in vitro cytotoxicity of this nanomaterial and used this method to quantify and assess their nanotoxicity. Given the behavior of AgNPs in the environment, toxicity may be affected by differences in their physico-chemical properties (size, agglomeration rate, adsorption on humic acid) and toxicity-inhibiting properties. We investigated the role of cells in absorbing AgNPs through agglutination and adsorption kinetics. It was found that the agglomeration and adsorption of AgNPs with sizes less than 14 nm were higher than that of AgNPs with sizes of 90 nm and 140 nm. For AgNPs with sizes of 90 nm and 140 nm, adsorption is significantly greater than agglomeration. It is noteworthy that under the normal concept, the normal concept that smaller and easier AgNPs are absorbed may be wrong under the influence of abiotic factors.