论文部分内容阅读
本文利用流式细胞仪研究了雷公藤总甙对人Ｔ细胞亚群及对ＩＬ－２产生和ＩＬ－２ＲＴａｃ链表达的影响。结果证实Ｔ＿Ⅱ可明显抑制ＰＨＡ刺激的Ｔ细胞ＩＬ－２ＲＴａｃ链表达，抑制ＩＬ－２产生，且均有剂量依赖关系。Ｔ＿Ⅱ对Ｔ细胞亚群中ＣＤ细胞和ＣＤ细胞从Ｇ＿０／Ｇ＿１期到Ｓ＋Ｇ＿２／Ｍ期均有明显的抑制作用。但两者受抑制的程度不同，其中对ＣＤＴ细胞的抑制作用更强。提示雷公藤总甙治疗自身免疫病的机制之一可能与其抑制ＩＬ－２自泌环路，降低体内活化的Ｔ细胞数量，调整Ｔ细胞亚群比例，从而恢复异常的免疫状态有关。
In this paper, flow cytometry was used to study the effects of Tripterygium wilfordii on human T cell subsets and the production of IL-2 and the expression of IL-2RTac chain. The results confirmed that T_II can significantly inhibit PHA-stimulated T cell IL-2RTac chain expression and inhibit IL-2 production in a dose-dependent manner. T_II significantly inhibited CD and CD cells in T cell subsets from the G 0 /G 1 phase to the S + G 2 /M phase. However, both are inhibited to different extents, among which the inhibitory effect on CDT cells is stronger. It is suggested that one of the mechanisms of treatment of autoimmune diseases by Tripterygium wilfordii may be related to its inhibition of IL-2 autocrine loop, lowering the number of activated T cells in vivo, adjusting the ratio of T cell subsets, and thus restoring abnormal immune status.