THERAPEUTIC EFFECTS OF PHOTOSENSITIZERS IN COMBINATION WITH LASER AND ACNU ON AN IN VIVO OR IN VITRO

来源 :Chinese Medical Journal | 被引量 : 0次 | 上传用户:rainbow_qu2009
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This study investigated the independent and combined effects of photodynamic therapy (PDT), laser photodynamic hyperthermia (LPDH) and 1-(4-amino-2-methyl-5-pyrimidinyl) methy1-3-(2-chloroethyl) -3-nitrosourea hydrochloride (ACNU) in a rat 9L induced gliosarcoma model. The mortality rate (MR60), mean survival time (MST60), and increasing life span (ILS60) within 60 days were determined to evaluate the therapeutic effect in vivo. The MR60 and MST60 of the gliosarcoma tumor control were 100% and 16.2 days. The ILS60s of PDT and ACNU were 72.84% and 49.81%, respectively, but MR60 of both were 86.72%. All combined treatments produced significantly prolonged survival (P<0.01). The combined effects of LPDH and ACNU, MR60, MST60, and ILS60 were 60%, 43 days, and 165.4%, respectively. The ILS60 of PDT+ACNU (96.48%) and PDT+LPDH (98.58%) also indicated a synergistic or additive effect. The survival fraction and synthetic rate of DNA, RNA, and protein of glioma 9L tumor cells in vitro after single treatment of PD This study investigated the independent and combined effects of photodynamic therapy (PDT), laser photodynamic hyperthermia (LPDH) and 1- (4-amino-2-methyl-5- pyrimidinyl) methy1-3- (2-chloroethyl) The mortality rate (MR60), mean survival time (MST60), and increasing life span (ILS60) within 60 days were determined to evaluate the therapeutic effect in vivo. The MR60 and MST60 (ACNU) in a rat 9L induced gliosarcoma model. of the gliosarcoma tumor control were 100% and 16.2 days respectively. The combined ILs60s of PDT and ACNU were 72.84% and 49.81% effects of LPDH and ACNU, MR60, MST60, and ILS60 were 60%, 43 days, and 165.4%, respectively. The ILS60 of PDT + ACNU (96.48%) and PDT + LPDH . The survival fraction and synthetic rate of DNA, RNA, and protein of glioma 9L tumor cells in vitro after sing le treatment of PD
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