论文部分内容阅读
目的:研究中国朝鲜族肾移植术后患者服用环孢素A(CsA)的谷浓度与多药耐药基因(MDR1)C3435T多态性的相关性。方法:选择中国朝鲜族同种异体肾移植术后患者75例,采用聚合酶链反应(PCR)扩增MDR1C3435T基因,利用DNA限制性片段长度多态性(RFLP)方法对MDR1C3435T基因进行分型,结合口服CsA的谷浓度,判断二者是否存在关联。结果:75例患者中,C/C型31例(41.3%),C/T型37例(49.3%)和T/T型7例(9.3%)。C3435T基因多态性与CsA谷浓度差异无统计学意义(P>0.05)。结论:C3435T基因多态性可能不是影响中国朝鲜族肾移植术后患者CsA谷浓度的主要因素。
Objective: To study the relationship between the trough concentration of cyclosporin A (CsA) and the multidrug resistance gene (MDR1) C3435T polymorphism in Korean patients after renal transplantation. Methods: Seventy - five Korean patients with renal allograft were enrolled. The MDR1C3435T gene was amplified by polymerase chain reaction (PCR) and the genotypes of MDR1C3435T were genotyped by restriction fragment length polymorphism (RFLP) Combined with the trough concentration of oral CsA, to determine whether there is correlation between the two. Results: Among the 75 patients, 31 (41.3%) were C / C, 37 (49.3%) C / T and 7 (9.3%) T / T. There was no significant difference between C3435T gene polymorphism and CsA trough concentration (P> 0.05). Conclusion: C3435T gene polymorphism may not be the main factor affecting the CsA trough concentration in Chinese patients after renal transplantation.