论文部分内容阅读
在碱性介质中,K3[Fe(CN)6]与鲁米诺产生化学发光反应,盐酸阿霉素对该体系的发光强度具有显著的抑制作用,且光信号的减小值(△I)与盐酸阿霉素的浓度在一定的范围内成正比。基于此建立了一种流动注射化学发光法测定盐酸阿霉素的新方法。在最优实验条件下,该方法的线性范围为0.4～2.5μg/mL,检出限为2.9×10-2μg/mL。对1.2μg/mL的盐酸阿霉素连续平行测定11次,其相对标准偏差为2.1%。该方法用于血浆中盐酸阿霉素的分析,回收率在98.4%～102.0%之间。
In basic medium, K3 [Fe (CN) 6] reacted with luminol to produce chemiluminescence. Adriamycin hydrochloride had a significant inhibitory effect on the luminescence intensity of the system, and the decrease of optical signal (△ I) And doxorubicin hydrochloride concentration is proportional to a certain range. Based on this, a new method for the determination of doxorubicin hydrochloride by flow injection chemiluminescence was established. Under the optimal experimental conditions, the linear range of this method was 0.4 ~ 2.5μg / mL with the detection limit of 2.9 × 10-2μg / mL. The relative standard deviation was 2.1% for the continuous parallel determination of doxorubicin hydrochloride 1.2 μg / mL 11 times. The method is used for the analysis of doxorubicin hydrochloride in plasma with the recoveries between 98.4% and 102.0%.