杂交瘤与单克隆抗体

• 作 者: James W Goding 杜佩英
• 出 处: 国外医学(免疫学分册)
• 发表日期: 1981年06期
• 关键词: 杂交瘤 骨髓瘤细胞 单克隆抗体 抗体分泌细胞 Kohler 饲养细胞 血清学 脾细胞 抗体 培养液

1975年Kohler与Milstein证明了正常抗体分泌细胞与骨髓瘤细胞触合而成的单克隆能够长期存活。“杂交瘤”方法使血清学进入了一个新的精确的时代。目前,杂交瘤技术已十分稳定,常用的方法有多种。在本文中,作者总结了主要的方法,试图为选择这些方法提供依据。

In 1975, Kohler and Milstein demonstrated that monoclonal antibodies made by normal antibody-secreting cells and myeloma cells can survive for a long time. The “hybridoma” approach has brought serology into a new era of precision. At present, hybridoma technology has been very stable, a variety of commonly used methods. In this article, the author summarizes the main methods and attempts to provide a basis for choosing these methods.