【摘 要】
：
The HsaI restriction enzyme from the embryos of human, Homo sapiens, has been isolated with both the tissue extract and nnelear extract. It proves to be an unu
【机 构】
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Institute of Biophysics, Academia Sinica, Beijingrrrrrrrrn,Institute of Biophysics, Academi
【出 处】
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Science in China,Ser.B
论文部分内容阅读
The HsaI restriction enzyme from the embryos of human, Homo sapiens, has been isolated with both the tissue extract and nnelear extract. It proves to be an unusual enzyme, clearly related functionally to Type Ⅱ endonuclease. HsaI seems to be an isoschizomer of EcoRI, but it has a distinctive property of elation, differing from EeoRI. Upon SDS-polyacrylamide gel electrophoresis, the enzyme preparation showed Coomassie blue staining bands, having the molecular weight of 65,000 and 22,000 daltons in size, respectively.
The HsaI restriction enzyme from the embryos of human, Homo sapiens, has been isolated with both the tissue extract and nnelear extract. It proves to be an unusual enzyme, clearly related functionally to Type II endonuclease. HsaI seems to be an isoschizomer of EcoRI, After it has a distinctive property of elation, differing from EeoRI. Upon SDS-polyacrylamide gel electrophoresis, the enzyme preparation showed Coomassie blue staining bands, having the molecular weight of 65,000 and 22,000 daltons in size, respectively.
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