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目的 研究急性肝衰竭大鼠肠道菌群及内毒素的动态变化。 方法 腹腔注射半乳糖胺建立急性肝衰竭大鼠模型。40只SD大鼠随机分为A组(对照组)10只;B组12只,C组18只(均为肝衰竭大鼠)。实验开始时(A组)、造模后24 h(B组)和48 h(C组)分别处死各组大鼠并检测肝功能,定性、定量分析空肠、回肠及结肠菌群,定量测定门静脉、右心室血,回肠及结肠内毒素。 结果 肝功能检测显示:B组大鼠的肝脏损伤最为严重;与B组相比,C组大鼠的肝功能开始好转。肠道菌群分析显示:B组大鼠肠道内肠杆菌科细菌显著增加(空肠、回肠间,P<0.01;结肠间,P<0.05)、乳酸杆菌下降(P<0.01);与B组相比,C组空肠和结肠内的肠杆菌科细菌出现下降(P<0.05)、乳酸杆菌增加,以空肠为显著(P<0.05)。内毒素测定表明B组回肠内毒素增加(P<0.05);C组空肠和回肠内毒素继续增高与对照组差异有显著性(P<0.01);门静脉内内毒素在B组最高,与A、C两组比较差异有显著性(P<0.01)。 结论 急性肝衰竭大鼠肠道存在菌群失调、肠杆菌科细菌过度生长,菌群失调程度与肝损伤程度有关;肠道菌群失调伴有回肠和结肠内内毒素升高;门静脉内毒素的增加与肠道菌群失调有关
Objective To study the dynamic changes of intestinal flora and endotoxin in rats with acute hepatic failure. Methods The model of acute liver failure was established by intraperitoneal injection of galactosamine. Forty SD rats were randomly divided into A group (control group) 10; B group 12, C group 18 (all liver failure rats). At the beginning of the experiment (group A), rats in each group were sacrificed at 24 h (group B) and 48 h (group C), and liver function was tested. The jejunum, ileum and colon flora were determined qualitatively and quantitatively. , Right ventricular blood, ileum, and endotoxin in the colon. Results Liver function tests showed that the liver injury in group B was the most serious. Compared with group B, the liver function of group C began to improve. Intestinal flora analysis showed that the number of enterobacteriaceae bacteria in group B was significantly increased (jejunum, ileum, P <0.01; between colon, P <0.05) and lactobacillus decreased (P <0.01) Compared with the control group, the Enterobacteriaceae in the jejunum and colon decreased in group C (P <0.05), lactobacillus increased significantly in jejunum (P <0.05). The level of endotoxin in group B was significantly higher than that in control group (P <0.01), and the level of endotoxin in group B was the highest (P <0.05) The differences between the two groups were significant (P <0.01). Conclusion The intestinal flora of acute hepatic failure in rats is imbalanced, the overgrowth of bacteria in Enterobacteriaceae and the degree of flora are related to the degree of hepatic injury. The imbalance of intestinal flora is accompanied by the increase of endotoxin in the ileum and colon, Increased and intestinal flora related