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以往研究证实超敏反应(HR)中的细胞程序化死亡(PCD)是植物对病毒的有效防御手段.但目前对系统获得性抗性反应(SAR)中的PCD至今还知之甚少.通过烟草花叶病毒(tobacco mosaic virus,TMV)-番茄(Lycopersicon esculentum)互作系统,发现TMV的局部侵染能够诱导番茄非接种部位的细胞死亡,但这些部位的酶联免疫吸附实验(ELISA)中未发现有病毒;且发生死亡的细胞都表现以下的生物学及分子学特征:染色质浓缩,形成团块,并于细胞核边缘分布;细胞核的TUNEL检测呈阳性;DNA ladder检测出现梯状条带;线粒体和叶绿体结构破毁;液泡膜和质膜发生收缩和降解等.结果说明TMV对番茄叶片的局部性侵染,在病毒不发生扩散的情况下,能引起根尖、茎尖和非接种叶片中的系统性PCD反应,上述组织中的这种反应在发生时间与同步性上不同,且在诱发原因和表现形式上与HR-PCD和SAR存在差异.
Previous studies have demonstrated that programmed cell death (PCD) in hypersensitivity (HR) is an effective defense against viruses by plants, but so far little is known about PCD in systemic acquired resistance (SAR) Tobacco mosaic virus (TMV) - Lycopersicon esculentum interaction system, we found that local infection with TMV induced cell death in non-inoculated tomato plants. However, in these sites, the enzyme-linked immunosorbent assay (ELISA) Found that the virus; and the death of the cells have shown the following biological and molecular characteristics: chromatin condensation, the formation of lumps and distribution in the nucleus edge; nuclear TUNEL test was positive; DNA ladder detection of ladder-like bands; The structure of mitochondria and chloroplast were destroyed, the vacuole and plasma membrane were contracted and degraded, etc. The results showed that TMV could locally infect tomato leaves and could induce root tips, shoot tips and non-inoculated leaves when the virus did not spread In the systemic PCD reaction in the above-mentioned tissue in the timing and timing of this reaction is different, and in the causes and manifestations of induction and HR-PCD and SAR differences exist.