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将已合成的密度感应拮抗剂呋喃C-30用BHI培养基配制至终浓度分别为2.0、4.0μg/mL,再加入新鲜培养的变异链球菌液,37℃微需氧培养24h,在96孔板上形成体外生物膜,用MTT法检测生物膜的量。激光共聚焦显微镜观察不同浓度呋喃C-30处理后的生物膜结构。实验中以不含呋喃C-30的BHI培养基作为阴性对照。结果:实验发现呋喃C-30不会对变异链球菌的生长产生影响,但随着呋喃C-30的浓度增加,变异链球菌形成生物膜的量显著降低(P<
The synthesized density-sensing antagonist furan C-30 was formulated in BHI medium to a final concentration of 2.0 and 4.0 μg / mL, respectively, and freshly prepared Streptococcus mutans was added to culture at 37 ° C. for 24 h. An in vitro biofilm was formed on the plate and the amount of biofilm was measured by MTT method. The confocal laser scanning microscopy was used to observe the biofilm structure of furan C-30 treated with different concentrations. BHI medium without furan C-30 was used as a negative control in the experiment. Results: Furan C-30 did not affect the growth of Streptococcus mutans, but the amount of Streptococcus mutans formed biofilm significantly decreased with the increase of C-30 concentration (P <