论文部分内容阅读
目的探讨双孔钾离子通道TASK-1、TRAAK蛋白在精子中的表达及其在弱精子症发生机制中的作用。方法非连续密度梯度离心分离、纯化正常男性和弱精子症患者精子后,采用Western印迹技术从蛋白水平检测TASK-1、TRAAK的表达及差异。结果 TASK-1、TRAAK蛋白在正常男性和弱精子症患者精子中均有表达;其中,TASK-1蛋白在弱精子症患者精子中的表达量与正常男性没有明显差异(P>0.05),TRAAK蛋白在弱精子症患者精子中的表达明显低于常男性(P<0.05),这与我们前期的RT-PCR结果一致。结论 TRAAK在弱精子症患者精子中的表达下调可能是精子活力下降的原因之一。
Objective To investigate the expression of TASK-1 and TRAAK, a double-hole potassium channel, in sperm and their roles in the pathogenesis of asthenospermia. Methods After non-continuous density gradient centrifugation and purification of sperm from normal male and asthenospermia patients, Western blotting was used to detect the expression and difference of TASK-1 and TRAAK from the protein level. Results TASK-1 and TRAAK protein were expressed in sperms of normal male and asthenospermia patients. Among them, the expression of TASK-1 protein in sperms of asthenospermia patients was not significantly different from that of normal male (P> 0.05) The protein expression of sperm in asthenospermia patients was significantly lower than that of the normal males (P <0.05), which was consistent with our previous RT-PCR results. Conclusions The down-regulation of TRAAK expression in sperm of asthenospermia patients may be one of the reasons for the decrease of sperm motility.