论文部分内容阅读
目的探讨临床I期(cI期)非小细胞肺癌(nonsmall-cell lung cancer,NSCLC)中血管内皮生长因子-C(vascular endothelial growth factor-C,VEGF-C)过度表达、MLVD计数、淋巴结转移的关系。方法应用免疫组化S-P法检测67例cI期NSCLC患者癌组织、癌旁组织、20例正常肺组织中VEGF-C蛋白表达和计数D2-40标记的MLVD,结合临床病理淋巴结转移情况进行统计学分析。结果在NSCLC癌组织中,VEGF-C过度表达(阳性率81%),MLVD计数显著增高(23.4±6.9),均较癌旁组织和正常肺组织有显著差异(P<0.01);淋巴结转移N2组的癌组织中VEGF-C表达(阳性率100.0%)、MLVD计数(28.5±3.3)明显高于淋巴结阴性组的癌组织[阳性率35.7%,(10.6±2.2),P<0.01];另外淋巴结转移数量和程度与VEGF-C表达强度成正相关(rs=0.62,P<0.01)。结论VEGF-C在cI期NSCLC癌组织中过度表达与淋巴结转移密切相关,提示VEGF-C过度表达的cI期肺癌患者应该强化手术区域淋巴结清扫和术后必要的化疗、放疗等辅助治疗。
Objective To investigate the expression of vascular endothelial growth factor-C (VEGF-C) overexpression, MLVD and lymph node metastasis in non-small cell lung cancer (NSCLC) relationship. Methods Immunohistochemical SP method was used to detect the expression of VEGF-C protein in 67 cases of NSCLC patients with NSCLC, 20 cases of normal lung tissues and the count of D2-40 labeled MLVD. Statistical analysis was performed based on the clinical pathological lymph node metastasis analysis. Results Overexpression of VEGF-C (positive rate was 81%) and MLVD count were significantly higher in NSCLC tissues (23.4 ± 6.9) than in adjacent non-cancerous tissues and normal lung tissues (P <0.01) The positive expression rate of VEGF-C (positive rate 100.0%) and MLVD (28.5 ± 3.3) were significantly higher in the cancer group than in the negative group (35.7%, 10.6 ± 2.2, P <0.01) The number and extent of lymph node metastasis were positively correlated with the expression of VEGF-C (rs = 0.62, P <0.01). Conclusion The overexpression of VEGF-C in c-stage NSCLC tissues is closely related to lymph node metastasis, suggesting that patients with c-stage lung cancer with overexpression of VEGF-C should be assisted with regional lymph node dissection and necessary postoperative chemotherapy and radiotherapy.