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以日本福岛县林业试验场日本柳杉种子园的 24个无性系为材料,利用 PCR—RAPD法和同功酶分析法对日本柳杉无性系的识别方法进行了比较研究。结果显示:同功酶法和由7个标记构成的RAPD分析法不能完全识别24个无性系,用17个和27个标记构成的RAPD分析法识别这24个无性系是可能的;同功酶标记是一种基因型标记,具有稳定性高的特点,适合于分析遗传构造的相似程度,特别适合于群体遗传分析,但是,由于能获得清晰话带的同功酶种类有限,在用于个体识别上比较困难;RAPD标记是一种显性标记,稳定性较差,但只要反应体系和条件稳定,利用RAPD标记正确进行无性系识别是完全可能的。
A total of 24 clones of Cryptomeria japonica were used as materials in the Forestry Experimental Station of Fukushima Prefecture in Japan. The methods of PCR-RAPD and isoenzyme analysis were used to identify the clonal clones of Cryptomeria japonica. The results showed that isozymes and RAPD analysis consisting of seven markers could not completely identify 24 clones, and it was possible to identify these 24 clones using RAPD analysis consisting of 17 and 27 markers. Isozyme Marker is a genotype marker with high stability and is suitable for analyzing the similarities of genetic constructs and is particularly suitable for population genetic analysis. However, due to the limited number of isoenzymes that provide clear bands, Identification is more difficult; RAPD marker is a dominant marker, stability is poor, but as long as the reaction system and conditions are stable, the use of RAPD markers correctly clonal identification is entirely possible.