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对氮丙啶类化合物 TMD的亚慢性毒性及毒突变作用进行了研究。毒性试验按 10、2 0、40 mg/ kg三个剂量组给小鼠经口染毒 ,致突变试验选用 Ames试验、微核试验和精子畸形试验。结果显示 ,TMD无明显蓄积。染毒 35天 ,40 m g/ kg组 WBC显著降低 ,Hb在 2 0和 40 mg/ kg组有显著降低 ;而染毒 45天各剂量组WBC、Hb、PT均有显著降低。血清 GPT、BU N无明显变化。脏器系数中仅睾丸、卵巢与对照组间有极显著性差异 (P<0 .0 1) ,且呈剂量反应关系。病理检查发现雌性 2 0、40 m g/ kg组卵巢各级卵细胞减少 ,雄性各剂量组均出现睾丸曲细精管明显损害。Ames试验 TMD为强阳性物质。试验组微核率、精子畸形率比对照组有极显著增高 (P<0 .0 1)。表明 TMD对造血系统影响明显 ,对生殖细胞具有明显损害和遗传毒性。
The subchronic toxicity of TMD and its mutagenicity were studied. Toxicity test The mice were orally exposed to the doses of 10, 20 and 40 mg / kg. The Ames test, micronucleus test and sperm abnormality test were selected for the mutagenicity test. The results showed no significant accumulation of TMD. On the 35th day of exposure, the WBC in 40 m g / kg group decreased significantly, while the Hb decreased significantly in 20 and 40 mg / kg groups. However, the WBC, Hb and PT in each dose group decreased significantly at 45 days. Serum GPT, BU N no significant change. There was significant difference between the testis and ovary in the organ coefficient and the control group (P <0.01), and showed a dose-response relationship. Pathological examination found that female ovaries at all levels of 20, 40 m g / kg reduced the number of oocytes, and all the male testicular seminiferous tubules showed significant damage in each dose group. Ames test TMD is a strongly positive substance. The micronucleus rate and sperm abnormality rate in the experimental group were significantly higher than those in the control group (P <0.01). TMD obvious impact on the hematopoietic system has significant damage to germ cells and genotoxicity.