论文部分内容阅读
目的 探讨在低密度脂蛋白 (LDL)环境中 ,人近端肾小管上皮细胞 (HKC)纤溶酶原抑制物 (PAI 1)和组织纤溶酶激活因子 (tPA)异常与核转录因子 κB(NF κB)激活的关系 ,及洛伐他汀 (Lov)的改善作用。方法 体外培养HKC ,用LDL刺激HKC ,并与Lov共孵育 ,发色底物法检测细胞上清液中PAI 1和tPA活性 ,逆转录聚合酶链反应后 ,观察HKC合成PAI 1和tPAmRNA表达 ,在共聚焦显微镜下 ,检测NF κB的表达。结果 LDL可激活NF κB的亚单位P6 5 ,使HKC培养上清液中PAI 1活性增加 ,mRNA表达为对照组的 2 .5倍。tPA活性由对照组的 6 .2 2± 0 .5 2IU/ml降至LDL组的 4 .9± 0 .11IU/ml(P <0 .0 5 ) ,mRNA表达降低 ,Lov可部分或全部逆转LDL的作用 ,甲羟戊酸可部分阻遏Lov作用。结论 LDL影响PAI 1/tPA活性及mRNA表达 ,可能和NF κB激活有关 ,Lov可通过影响NF κB的激活 ,逆转LDL的作用。
Objective To investigate the relationship between aberrant PAI 1 and tPA in human proximal tubular epithelial cells (HKC) and nuclear factor kappa B (NF - κB) in low density lipoprotein (LDL) NF κB) activation, and lovastatin (Lov) to improve the role. Methods HKC was cultured in vitro, HKC was stimulated with LDL and incubated with Lov. The activity of PAI 1 and tPA in the cell supernatant was detected by chromogenic substrate method. After reverse transcriptase polymerase chain reaction, the expression of PAI 1 and tPA mRNA in HKC were observed, Under confocal microscope, the expression of NF KB was detected. Results LDL activated NF-κB subunit P6 5, which increased PAI1 activity in HKC culture supernatants and mRNA expression was 2.5-fold higher than that of control. The tPA activity decreased from 6.2 ± 0.25IU / ml in the control group to 4.9 ± 0.11IU / ml in the LDL group (P <0.05), and the mRNA expression decreased. Lov was partially or completely reversed LDL role, mevalonate can partially block the role of Lov. Conclusion LDL may affect PAI 1 / tPA activity and mRNA expression, which may be related to the activation of NF κB. Lov may reverse the effect of LDL by affecting the activation of NF κB.