论文部分内容阅读
用枯斑反应和酶联免疫吸附试验(ELISA)检查了感染SMV的六个大豆品种的叶、花、未成熟和成熟的去皮种子内的SMV浓度,并与种子传毒率做了相关分析。结果表明,不同品种间,叶片、未成熟种子内SMV浓度显著不同,(P<0.05),但与品种间在种子传毒率上的差异无相关性。不同品种间,花叶SMV浓度无明显差別。成熟的去皮种子内,群体病毒浓度与种子传毒率呈明显正相关,r=0.95(P<0.05)而与病种子群体内SMV浓度无关。因此认为,品种间在种子传毒率方面的差异不是由于其叶片、未成熟种子内病毒浓度不同所致,而是由于种胚和子叶在即将成熟或成熟时受SMV侵染率的高低所决定的。而后者可通过检查成熟的去皮种子群体内SMV浓度来估计。
The concentrations of SMV in leaves, flowers, immature and mature peeled seeds of six soybean infected SMVs were examined by a blot reaction and enzyme-linked immunosorbent assay (ELISA) . The results showed that the concentration of SMV in different varieties, leaves and immature seeds were significantly different (P <0.05), but there was no correlation with the difference in the rate of seed germination among varieties. There was no significant difference in SMV concentration among different varieties. In mature peeled seeds, the population virus concentration was positively correlated with the rate of seed germination, r = 0.95 (P <0.05) but not with the concentration of SMV in the diseased seed population. Therefore, it is considered that the difference in the rate of seed-to-seed transmission between cultivars is not due to the difference in virus concentration in leaves and immature seeds, but rather due to the prevalence of SMV infection in embryo and cotyledons as they mature or mature of. The latter can be estimated by examining the concentration of SMV in a mature peeled seed population.