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以野大麦（Ｈｏｒｄｅｕｍｂｒｅｖｉｓｕｂｕｌａｔｕｍ）的幼穗诱导愈伤组织，建立悬浮细胞系，利用胚性悬浮细胞系在不同的酶解条件和不同的酶解液中分离原生质体并进行培养，获得了细胞分裂，形成细胞团。
The calli were induced from the young ears of Hordeumbrevisubulatum and the suspension cell lines were established. The protoplasts were isolated and cultured under different enzymolysis conditions and different enzymatic hydrolysis solutions by using embryogenic suspension cell lines. The cell division, Formation of cell clusters.