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目的:探讨肺癌中XPD基因表达及其与微卫星不稳定(MSI)的关联情况。方法:采用微卫星分析和RT-PCR方法检测了5个微卫星标记在肺癌中的状况及XPD转录水平的表达。结果:27.16%(22/81)的肺癌出现至少1个微卫星的不稳定,但是XPD没有明显的mRNA表达下调。MSI与肺癌分期、分级、淋巴结转移和患者性别等参数之间无相关性,P>0.05。结论:XPD基因微卫星的不稳定是肺癌的频发事件,与XPD基因表达可能不存在必然联系。
Objective: To investigate the relationship between XPD gene expression and microsatellite instability (MSI) in lung cancer. Methods: The microsatellite markers and RT-PCR were used to detect the expression of five microsatellite markers in lung cancer and XPD transcription. RESULTS: There was at least 1 microsatellite instability in lung cancer in 27.16% (22/81) of the lungs, but no significant decrease in XPD mRNA expression. There was no correlation between MSI and lung cancer staging, grading, lymph node metastasis and patient gender, P> 0.05. Conclusion: The microsatellite instability of XPD gene is a frequent occurrence of lung cancer, which may not be related to the expression of XPD gene.