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目的研究4种新类型霍乱弧菌(VC)毒力协同调节菌毛(TCP)氨基酸序列进行生物信息学特征。方法基因序列及氨基酸序列利用DNAStar和BLAST进行分析。结果对4种新类型tcpA推测的氨基酸序列的α螺旋、β折叠、疏水性、抗原表位等特征进行分析,并与国外发现的4种类型比较,N末端约100个氨基酸序列的抗原表位等特征高度一致,而C末端约60个氨基酸序列的抗原表位等特征差别较大。在对tcpA基因扩增及测序时,扩增片段包括tcpB基因5#端部分基因及其启动子区,序列分析表明,不同类型tcpB的启动子区差别较大,不同类型tcpB5#端部分基因存在多个位点突变,但均未导致氨基酸变异。结论4种新类型TCP和国外发现的4种类型的C末端抗原表位差别较大,保护性抗原表位主要位于C末端,提示不同类型TCP抗原交叉保护性较弱。
Objective To study the bioinformatics characteristics of four new types of Vibrio cholera (VC) virulence co-regulating the amino acid sequence of pili (TCP). Methods The gene sequence and amino acid sequence were analyzed by DNAStar and BLAST. Results The amino acid sequences of α-helix, β-sheet, hydrophobicity and epitope of the four new types of tcpA deduced amino acids were analyzed. Compared with the four foreign types, the N-terminal epitope of about 100 amino acids And other features are highly consistent, while the C-terminal about 60 amino acid sequence characteristics such as antigen epitopes vary widely. In the amplification and sequencing of tcpA gene, the amplified fragment includes the 5 # terminal part of tcpB gene and its promoter region. Sequence analysis shows that the promoter regions of different types of tcpB differ greatly, and some tcpB5 # A number of site mutations, but did not lead to amino acid variation. Conclusion The four new types of TCP and the four types of C-terminal epitopes found abroad are quite different. The protective epitope is mainly located at the C-terminus, suggesting that the cross-protection of different types of TCP antigens is weak.