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目的：观察树是否可成为感染人ＨＢＶ的动物模型。方法：应用免疫组化及原位杂交技术，对实验组及对照组树肝组织进行研究分析。结果：应用羊抗－ＨＢｓ多抗检测树肝组织中的ＨＢｓＡｇ时，出现假阳性反应，阳性物为非目的抗原，应用生物素标记的ＨＢＶＤＮＡ探针进行肝组织原位杂交时，出现非特异性的显色反应，结论：提示在动物模型实验中，应设立严格的对照实验和应用不同反应系统进行校对考核。
Objective: To observe whether the tree can become an animal model of HBV infection. Methods: The liver tissues of experimental group and control group were studied by immunohistochemistry and in situ hybridization. Results: The detection of HBsAg in the liver tissue by goat anti-HBs polyketide showed a false positive reaction, and the positive was non-target antigen. When using biotin-labeled HBVDNA probe for in situ hybridization of liver tissues, nonspecific Color reaction, Conclusion: Tip animal model experiments should be set up strict control experiments and the application of different reaction systems proofreading examination.