论文部分内容阅读
采用卫生部《化学品毒性技术鉴定规范》中试验方法对2,4-二硝基苯甲醚(DNAN)的致突变性进行研究。Ames试验结果显示,DNAN在每皿200~2500μg剂量范围引起T98菌株(加S9)回变菌落数增加(P<0.05),且超过溶剂对照菌落数的2倍,存在剂量-效应关系;微核试验结果显示,DNAN在6~22 mg/kg剂量范围内不引起小鼠骨髓嗜多染红细胞微核率增加。提示DNAN在Ames试验中有致突变作用,但在小鼠体内试验中未发现致突变作用,为可疑诱变剂。
The mutagenicity of 2,4-dinitroanisole (DNAN) was studied by the test method of “Identification Standard of Chemical Toxicity” of the Ministry of Health. The results of Ames test showed that DNAN increased the number of colony of T98 strain (plus S9) (P <0.05) and exceeded the control colony count of 2 times in a dose-dependent manner in the range of 200 ~ 2500μg per dish. The results showed that, DNAN in the dose range of 6 ~ 22 mg / kg did not cause increased micronuclei in mouse bone marrow polychromatic erythrocytes. Tip DNAN mutagenic effect in the Ames test, but no in vivo tests in mice found mutagenic effect, as a suspect mutagen.