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We deduce the surface temperature distribution generated by the inner point heat source in biological tissues and propose a graphic method to retrieve the depth of the point heat source.The practical surface temperature distribution can be regarded as the convolution of the temperature distribution of the inner point heat source with the heat source shape function.The depth of an abnormal heat source in biological tissues can be retrieved by using the graphic method combined with the blind deconvolution scheme.Surface temperature distribution of the biological tissues is closely related to the neighboring metabolic heat production,blood circulation in an organism and environmental temperature.[1] The abnormal metabolic performances of a local region in biological tissue imply malignant changes occurring,which can be distinguished from the variance of surface temperature.Mode development of thermal infrared (TIR) imaging has made the surface temperature measurement of biological tissue easier.Nowadays,several types of tumors,e.g.skin or breast can be recognized with TIR imaging.[2] The diagnostics with TIR imaging require more experienced operators and can not accurately ascertain the site of pathological changes,which limits the value of this technology.Therefore ascertaining the depth of inner heat source in biological body has the extremely important clinical value.