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目的：探讨蛋白激酶Ｃ（ＰＫＣ）抑制剂Ｓｔａｕｒｏｓｐｏｒｉｎｅ（ＳＴ）诱导ＣＮＥ－２Ｚ细胞凋亡时，对细胞周期的选择性和对ＤＮＡ含量及细胞周期的动态影响。方法：以ＰＫＣ催化区抑制剂ＳＴ作用于ＣＮＥ－２Ｚ细胞不同时间后，收集细胞进行ＰＩ染色和流式细胞仪检测。结果：ＳＴ以２×１０－６ｍｏｌ／Ｌ终浓度分别作用至３，６，１２，２４ｈ后，发现Ｇ２期百分比分别为３０．４３±７．１６、３０．８３±６．２５、４２．４３±３．９８和７１．８０±７．５５，均较对照组１２．０８±３．４９增高明显。比较差异有显著性（Ｐ＜０．０１）；而Ｇ１期在３，６，１２，２４ｈ分别为３４．７０±１．５６，３７．０３±６．４７，１６．６３±１．７７和１５．３０±５．４６，均较对照组６２．５６±６．５７明显降低，比较差异有显著性（Ｐ＜０．０１）。亚二倍体峰百分比在１２、２４ｈ分别为３７．１０±７．２１和４３．７２±６．７３，均较对照组９．８１±１．７８显著增加（Ｐ＜０．０１）；而１２ｈ以前该峰增加不明显。结论：ＳＴ在终浓度为２×１０－６ｍｏｌ／Ｌ诱导ＣＮＥ－２Ｚ细胞至１２ｈ后即可出现明显的ＤＮＡ含量降低的凋亡细胞，对Ｇ２期细胞敏感而使之逐渐阻滞，且存在?
AIM: To investigate the cell cycle selectivity, DNA content and cell cycle dynamics of CNE-2Z cells induced by protein kinase C (PKC) inhibitor Staurosporine (ST). Methods: After treated with STP, a PKC inhibitor, for different time periods, cells were collected for PI staining and flow cytometry. Results: After treated with 2 × 10-6mol / L of ST for 3, 6, 12 and 24 hours respectively, the percentage of G2 phase was found to be 30.43 ± 7.16, 30.83 ± 6.25 and 42.43 ± 3.98 and 71.80 ± 7.55, all higher than the control group 12.08 ± 3.49 significantly increased. (P <0.01), while the G1 phase was 34.70 ± 1.56, 37.03 ± 6.47, 16.63 ± 1.77 at 3, 6, 12 and 24 hours respectively 15.30 ± 5.46, respectively, compared with the control group, 62.56 ± 6.57, the difference was significant (P <0.01). The percentage of sub-diploid peak was 37.10 ± 7.21 and 43.72 ± 6.73 at 12 and 24 hours, respectively, which were significantly higher than those in control group (9.81 ± 1.78, P <0.01) The peak increased slightly before 12h. Conclusion: The apoptotic cells with significant DNA content decrease after ST induced by 2 × 10-6mol / L of CNE-2Z cells for 12h, which is sensitive to G2 cells and gradually blocked.