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最近已在哺乳类动物脑内克隆出两种新的脑内特殊的Na+依赖的无机磷酸转运体 ,它们均属于囊泡膜谷氨酸转运体 ,被分别命名为VGluT1(Vesicularglutamatetransporteroftype1)和DNPI (differentiation associatedNa+ dependentinorganicphosphatecotransporter)。本研究应用免疫荧光组织化学三重染色技术 ,在激光共聚焦显微镜下观察了大鼠三叉神经中脑核 (Vme)内VGluT1样和DNPI样以及谷氨酸脱羧酶 (GAD)样阳性末梢与GABAA受体α1亚单位 (GABAARα1)样阳性神经元之间的联系。结果显示 :①几乎所有的Vme神经元均呈GABAA受体α1亚单位样免疫阳性 ,吻尾方向在其全长出现 ,这些神经元绝大多数为大的假单极神经元 (直径为 2 5～ 5 0μm) ,但也有小部分为直径小于 2 5 μm的神经元。②大量的VGluT1样和DNPI样免疫阳性的神经纤维和末梢广泛分布于Vme内 ,其中DNPI样阳性纤维和末梢的分布密度高于VGluT1;同时还观察到GAD样阳性纤维和末梢也密集分布于Vme内。③VGluT1样、DNPI样和GAD样免疫阳性终扣分别包绕在GABAA受体α1亚单位样阳性Vme神经元胞体周围 ,并与之形成密切接触。以上结果提示 :Vme神经元在介导面口部本体感觉信息的传递中 ,可能同时接受中枢其他来源的谷氨酸能和GABA能末梢的调控 ,其中GABA能的投射末梢?
Recently, two new brain-specific Na + -dependent inorganic phosphate transporters have recently been cloned in mammalian brains, all of which belong to the vesicular glutamate transporter and are named VGluT1 (Vesicular glutamate transporter of type 1) and DNPI (differentiation associatedNa + dependentinorganicphosphatecotransporter). In this study, immunofluorescence histochemical triple staining technique was used to observe VGluT1-like and DNPI-like and glutamate decarboxylase (GAD) -like positive terminals and GABAA-like receptors in the rat trigeminal nucleus (Vme) under confocal laser scanning microscope Body α1 subunit (GABAARα1) -like positive neurons. The results showed that: (1) Almost all Vme neurons were immunopositive in the GABAA receptor α1 subunit, and the tail direction appeared in its full length. Most of these neurons were large pseudopolar neurons ~ 50μm), but a few of them are neurons less than 25μm in diameter. (2) A large number of VGluT1-like and DNPI-like immunoreactive nerve fibers and terminals were widely distributed in Vme. The distribution density of DNPI-like positive fibers and terminals was higher than that of VGluT1. GAD-like positive fibers and terminals were also observed to be densely distributed in Vme Inside. (3) VGluT1-like, DNPI-like and GAD-like immunoreactive terminals were wrapped around and closely contacted with the Gamma-1 subunit-like positive Vme neurons of GABAA receptor. The above results suggest that: Vme neurons may mediate the sensory information transmission of the oral and maxillary noumenal receptors, and may also accept the regulation of glutamatergic and GABAergic terminals from other sources in the central nervous system. Which GABAergic projection terminal?