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本文报道在pH9.9条件下,用甲苯萃取尿样,经与手性荧光试剂S-(+)氯化苯恶洛芬衍生化及薄层色谱分离后,以荧光分析法测定三种肾上腺受体阻滞剂——美多心安、心得平和普萘洛尔的对映体,该方法操作简便、快速.作者进行了对照化合物,(普萘洛尔)的合成,衍生物的荧光特性,萃取,衍生化反应,色谱分离及检测条件,标准曲线和复演性,代谢产物的干扰,试剂中R-氯化苯恶洛芬的定量,萃取过程中有否外消旋化的验证,结果的校正以及2名健康受试者服药后的尿样测定等试验.结果表明,美多心安、心得平、普萘洛尔衍生物的激发波长(λex)和发射波长(λem)分别为313和360nm,313和364nm,313和359nm.色谱图表明均得基线分离,以普萘洛尔为例,尿样通过萃取,衍生化后测得的衍生化反应率达95%,绝对回收率为78%.每毫升尿中含美多心安<40μg、心得平<30μg、普萘洛尔<30μg时,标准曲线均
In this paper, urine samples were extracted with toluene at pH9.9. After derivatized with chiral fluorescent reagent S - (+) - benoxaprofen and thin-layer chromatography, the three adrenal receptors The body blocker-Metoprolol, enantiomer of propranolol peacefully, the method is simple and fast, the author of the control compound, (propranolol) synthesis, the fluorescence properties of derivatives, extraction , Derivatization, chromatographic separation and detection conditions, standard curve and reverberation, interference of metabolites, quantification of R-chlorobenzofuran in reagents, validation of racemization during extraction, results of Calibration and urine test of two healthy subjects after taking the drug.The results showed that the excitation and extinction wavelengths (λex and λem) of metoprolol and propranolol derivatives were 313 and 360 nm, respectively , 313 and 364nm, 313 and 359nm. Chromatograms showed baseline separation, taking propranolol as an example. After derivatisation of urine samples, the derivatization rate was 95% and the absolute recovery was 78% Per milliliter of urine containing the United States more than the safety of <40μg, the level of peace <30μg, propranolol <30μg, the standard curve