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马铃薯干腐病是由镰刀菌导致的马铃薯窖储真菌病害之一,获得高质量镰刀菌的DNA是建立马铃薯干腐病分子检测的前提。本试验采用改良SDS法和2×CTAB法对黑龙江省马铃薯干腐病五种镰刀菌的DNA提取方法进行了比较。结果表明,在腐皮镰刀菌(Fusarium solani(Mart.))和拟丝孢镰刀菌(Fusarium trichothecioides Wollenw)的DNA提取过程中,改良SDS法优于2×CTAB,获得的DNA含量较高、条带较亮、纯度较高、完整性较好,而对于燕麦镰刀菌(Fusarium avenaceum(Corda&Fr.)Sacc)、接骨木镰刀(Fusarium sambucinum Fuckel)和拟枝镰刀菌(Fusarium sporotrioides Sherb)的DNA提取两种方法差异不大,但获得的DNA都可以很好的应用到后面的分子实验过程中,此试验获得的结果可以为马铃薯干腐病致病菌的分子生物学研究提供基础。
Dry-rot disease of potato is one of the fungal diseases of potato cellar caused by Fusarium, and obtaining high-quality Fusarium DNA is the prerequisite for molecular detection of dry rot of potato. In this study, DNA extraction methods of five Fusarium species of potato stem rot disease in Heilongjiang Province were compared using improved SDS method and 2 × CTAB method. The results showed that the modified SDS method was superior to 2 × CTAB in DNA extraction of Fusarium solani (Mart.) And Fusarium trichothecioides Wollenw, The bands are brighter, of higher purity and of better integrity, whereas for DNA extraction of two Fusarium avenaceum (Corda & Fr.) Sacc, Fusarium sambucinum Fuckel and Fusarium sporotrioides Sherb However, the obtained DNA can be well applied to the subsequent molecular experiments. The results obtained in this experiment can provide the basis for the molecular biology study of potato dry rot pathogens.