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目的观察碱性成纤维细胞生长因子（ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ｂＦＧＦ）对牛眼晶体上皮细胞的作用。方法首先进行牛眼晶体上皮细胞原代与传代培养；在第２代培养细胞中添加ｂＦＧＦ，浓度１０－２～１０２ｎｇ／ｍｌ，借助甲基噻唑基四唑（ｍｅｔｈｙｌｔｈｉａｚｏｌｙｌｔｅｔｒａｚｏｌｉｕｍ，ＭＴＴ）测定方法观察ｂＦＧＦ对晶体上皮细胞增殖的影响。结果牛眼晶体上皮细胞体外具有易于贴附、细胞融合较快以及生长迅速等特点。添加ｂＦＧＦ后，可促进晶体上皮细胞的增殖，尤其是１０２ｎｇ／ｍｌ显示出明显的促增殖作用。结论ｂＦＧＦ在促进白内障术后晶体上皮细胞的增殖及后囊混浊上起了重要作用。
Objective To observe the effect of basic fibroblast growth factor (bFGF) on bovine lens epithelial cells. Methods Primary and subculture of bovine lens epithelial cells was performed. BFGF was added to the second generation of cultured cells at a concentration of 10-2 ~ 102ng / ml, and the bFGF was detected by methyl thiazolyl tetrazolium (MTT) assay Effect of proliferation of crystal epithelial cells. Results Bovine lens epithelial cells in vitro with easy attachment, rapid cell fusion and rapid growth characteristics. The addition of bFGF promoted the proliferation of crystal epithelial cells, especially 102ng / ml. Conclusion bFGF plays an important role in promoting the proliferation of lens epithelial cells and posterior capsule opacification after cataract surgery.