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目的检测新疆维吾尔族妇女宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)和宫颈癌中C-myc和hMLH1蛋白表达,并探讨其临床意义。方法应用免疫组化SP法检测C-myc与hMLH1在慢性宫颈炎、宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)和宫颈癌组织中的表达水平,并探讨宫颈癌组织中C-myc和hMLH1蛋白表达与各临床病理特征的关系。结果 C-myc在慢性宫颈炎、CIN、宫颈癌中的阳性表达率分别为26.7%(8/30)、50.0%(30/60)和69.2%(36/52),差异有统计学意义(P<0.05)。hMLH1在慢性宫颈炎、CIN和宫颈癌组织中的阳性表达率依次为66.7%(20/30)、56.7%(34/60)和30.7%(16/52)差异有统计学意义(P<0.05)。在宫颈癌组织中,C-myc蛋白与肿瘤分化程度、临床分期以及有无淋巴结转移相关;hMLH1蛋白表达下调与宫颈癌分化程度及是否有淋巴结转移密切相关,表达差异均有统计学意义(P<0.05)。结论 C-myc和hM-LH1蛋白与宫颈癌的发生、发展可能相关,二者联合检测可能为宫颈癌的早期诊断和治疗提供新的方向。
Objective To detect the expression of C-myc and hMLH1 protein in cervical intraepithelial neoplasia (CIN) and cervical cancer in Uigur women in Xinjiang and to explore its clinical significance. Methods The expressions of C-myc and hMLH1 in chronic cervicitis, cervical intraepithelial neoplasia (CIN) and cervical cancer tissues were detected by immunohistochemical SP method. The expressions of C-myc and hMLH1 in cervical cancer tissues Relationship between protein expression and clinicopathological features. Results The positive rates of C-myc in chronic cervicitis, CIN and cervical cancer were 26.7% (8/30), 50.0% (30/60) and 69.2% (36/52), respectively. The difference was statistically significant ( P <0.05). The positive rates of hMLH1 in chronic cervicitis, CIN and cervical cancer were 66.7% (20/30), 56.7% (34/60) and 30.7% (16/52), respectively ). In cervical cancer, C-myc protein was correlated with tumor differentiation, clinical stage and lymph node metastasis. The down-regulation of hMLH1 protein was closely related to the differentiation of cervical cancer and lymph node metastasis (P <0.05). Conclusion The expressions of C-myc and hM-LH1 may be related to the occurrence and development of cervical cancer. The combined detection of C-myc and hM-LH1 may provide a new direction for the early diagnosis and treatment of cervical cancer.