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为了探讨神经肽对心功能的调节，用６－羟多巴胺化学损毁大鼠心脏交感神经纤维后，观察了动物心电图的变化，而后用免疫组织化学法结合图像分析、放射免疫测定观测了窦房结区神经肽Ｙ（ＮＰＹ）及降钙素基因相关肽（ＣＧＲＰ）的变化；同时应用组织化学法结合图像分析、生化测定观测了窦房结区去甲肾上腺素（ＮＡ）及乙酰胆碱酯酶（ＡＣｈＥ）的变化。结果发现，静脉注射６－羟多巴胺５０ｍｇ／ｋｇ体重１周后，引起大多数动物心电图的明显改变：心率减慢，Ｐ－Ｒ间期及Ｑ－Ｔ间期延长。心脏窦房结区ＮＡ及ＮＰＹ含量明显减少，而ＡＣｈＥ活性显著增加、ＣＧＲＰ含量增加不明显。结果提示心脏交感神经纤维损毁后大鼠心电图的变化可能与心内多种神经递质的相互作用有关，神经肽在其中可能具有重要的调节作用。
In order to investigate the regulation of neuropeptide on cardiac function, 6-hydroxydopamine chemical damage to rat cardiac sympathetic nerve fibers was observed after the changes of animal electrocardiogram, and then using immunohistochemistry combined with image analysis, radioimmunoassay observed sinoatrial node Neuropeptide Y (NPY) and calcitonin gene-related peptide (CGRP) were detected by real-time quantitative real-time PCR. Meanwhile, histochemistry and image analysis were used to observe the changes of norepinephrine (NA) and acetylcholinesterase (AChE) )The change. The results showed that intravenous injection of 6-hydroxydopamine 50 mg / kg body weight for 1 week caused a significant change in most animals electrocardiogram: heart rate slowed, P-R interval and Q-T interval prolongation. The contents of NA and NPY in the sinus node were significantly decreased, while the activity of AChE was significantly increased while the content of CGRP was not significantly increased. The results suggest that changes of rat electrocardiogram after cardiac sympathetic nerve fiber damage may be related to the interaction of various neurotransmitters in the heart, and neuropeptide may play an important regulatory role in them.