A PRELIMINARY STUDY ON THE IMMUNODIAGNOSIS OF PARAGONIMIASIS——THE PHYSICO-CHEMICAL AND IMMUNOLOGICAL

• 作 者: 张夏英 孙家鑫 熊军 贺联印 高佩芝 李浩虹 钟惠澜
• 机 构: Beijing Tropical Medicine Research Institute,Beijing Tropical Medicine Research Institute,Beijing Tr
• 出 处: Science in China,Ser.B
• 发表日期: 1986年01期
• 关键词: crude preparing partially infected purified deviation LIAONING remaining prepare

We have now succeeded in preparing a partially purified antigen (Fr. 1). The positive rates of the indirect ELISA were 100% both for sera of proved human cases and dogs infected with Paragonimus westermani when Fr. 1 was used, whereas the positive rates of the test were 71.4% and 75% on sera from patients and infected dogs respectively when crude antigen was used. Moreover, it is also better than the crude antigen in specificity, so a more reliable test in immunodiagnosis of paragonimiasis is available in our laboratory.

We have now succeeded in preparing a partially purified antigen (Fr. 1). The positive rates of the indirect ELISA were 100% both for sera of proven human cases and dogs infected with Paragonimus westermani when Fr. 1 was used, but the positive rates of the test were 71.4% and 75% on sera from patients and infected dogs respectively when crude antigen was used. Moreover, it is also better than the crude antigen in specificity, so a more reliable test in immunodiagnosis of paragonimiasis is available in our laboratory .