论文部分内容阅读
通过对酸性非特异性酯酶(ANAE)标记人周血淋巴细胞方法的10项步骤对比,见到对分离的淋巴细胞以戊二醛弱固定,15％福尔马林钙(1.3％)复固定时,酶活性保存和胞核复染好。此福尔马林钙固定全血膜片,尚具有溶解红细胞的优点,尤其是固定以磷酸缓冲盐水稀释的血膜片,比血涂片更易使片上的红细胞溶去,而使淋巴细胞数目集中,易于计数。以国产重氮化副品红及0.5mg/ml的α-醋酸萘酯浓度的培育液,于37℃培育约2h,ANAE细胞分型辨别较好。共观察28例成人,血片淋巴细胞及分离的淋巴细胞的ANAE分型中,点型约占56％,弥散型约占25％,阴性型约占19％,两者计数结果近似。对同一个体分离的淋巴细胞与血片淋巴细胞做对比观察,见到点型减少不显著,弥散型明显增多,阴性型明显减少。
Comparison of 10 steps in the method of labeling human peripheral blood lymphocytes with acidic non-specific esterase (ANAE) showed that the isolated lymphocytes were fixed with glutaraldehyde weakly and 15% formalin calcium (1.3%) When enzyme activity is preserved and nuclei are well counterstained. The formalin-fixed whole blood patch still has the advantages of dissolving red blood cells, especially fixed with phosphate buffered saline diluted blood film than the blood smear easier to dissolve the red blood cells on the chip, leaving the number of lymphocytes focus , Easy to count. ANAE cells were well differentiated with domestic diazo-paired magenta and 0.5 mg / ml α-naphthyl acetate concentration culture solution at 37 ℃ for about 2 hours. A total of 28 cases of adult, blood film lymphocytes and lymphocytes isolated ANAE typing, point type accounted for about 56%, about 25% of diffuse, negative accounted for about 19%, the two count results are similar. On the same individual isolated lymphocytes and blood cells compared lymphocyte observation, to see the point type was not significantly reduced, significantly increased diffuse, negative significantly reduced.