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Objective: To investigate whether deletion ofchromosome 3 is involved in the carcinogenesis of primary glioblastoma multiforme (GBM) and to localize the possible common deletion region in the aforementionedchromosome. Methods: PCR based microsatellite polymorphism analyses were performed to detect loss of heterozygosity (LOH). Twenty-three loci on chromosome were examined in 20 cases of GBM. Fluorescence-labeled primers and Perkin Elmer 377 DNA Sequencer wereapplied. Results: 50% informative cases of GBM displayed LOH on chromosome 3. 50% of informative casesdisplayed LOH on 3q and 35% on 3p. 25.6% ofinformative loci showed LOH in our series, in whichfrequent LOH were observed in the chromosomal regionfrom loci D3S1614 (42.9%) to D3S1565 (35.3%) on 3q24-27 and at loci D3S1569 (35.3%) on 3q22-23 and D3S1289(33.3%) on 3p14.1-14.3. Conclusion: Loss of geneticmaterial on chromosome 3 may play an important part ithe tumorigenesis of GBM. The chromosomal regionsfrom loci D3S1614 to D3S1565 on 3q24-27 and at loci D3S1569 on 3q22-23 and D3S1289 on 3p14.1-14.3 are potential sites for novel tumor suppressor genes associated with GBM.
Objective: To investigate whether deletion of chromosome 3 is involved in the carcinogenesis of primary glioblastoma multiforme (GBM) and to localize the possible common deletion region in the previous Chromosome. Methods: PCR based microsatellite polymorphism analyzes were performed to detect loss of heterozygosity (LOH). Twenty-three loci on chromosomes were examined in 20 cases of GBM. Fluorescence-labeled primers and Perkin Elmer 377 DNA Sequencer were applied. Results: 50% informative cases of GBM displayed LOH on chromosome 3. 50% of informative casesdisplayed LOH on 3q and 35 % on 3p. 25.6% ofinformative loci showed LOH was observed in the chromosomal regionfrom loci D3S1614 (42.9%) to D3S1565 (35.3%) on 3q24-27 and at loci D3S1569 (35.3%) on 3q22- 23 and D3S1289 (33.3%) on 3p14.1-14.3. Conclusion: Loss of genetic material on chromosome 3 may play an important part ithe tumorigenesis of GBM. The chromosomal regions from loci D3S1614 to D3S 1565 on 3q24-27 and at loci D3S1569 on 3q22-23 and D3S1289 on 3p14.1-14.3 are potential sites for novel tumor suppressor genes associated with GBM.