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目的探讨磷酸化磷虾肽(phosphorylated peptides from Antarctic krill,PP-AKP)对骨质疏松症大鼠血清炎症因子及骨吸收的影响。方法采用双侧去卵巢方法建立骨质疏松症大鼠模型,将大鼠随机分为假手术组,模型对照组,阳性对照组(阿仑膦酸钠,1 mg/kg·bw),PP-AKP低、高剂量组(400、800 mg/kg·bw)。连续灌胃90d,1/d。实验结束后,检测大鼠血清抗酒石酸酸性磷酸酶、组织蛋白酶K活性,和血清α-肿瘤坏死因子等相关炎症因子含量;检测尿液钙、磷和脱氧吡啶啉含量。结果 PP-AKP可显著抑制血清抗酒石酸酸性磷酸酶和组织蛋白酶K活性(P<0.01),显著降低尿液钙、磷和脱氧吡啶啉含量(P<0.01);显著降低大鼠血清α-肿瘤坏死因子、白细胞介素6、白细胞介素1β、一氧化氮含量(P<0.01)和环氧化酶2活性(P<0.01)。结论 PP-AKP可显著抑制骨质疏松症大鼠骨吸收,其作用机制可能与抑制炎症因子的分泌有关。
Objective To investigate the effects of phosphorylated peptides from Antarctic krill (PP-AKP) on serum inflammatory factors and bone resorption in rats with osteoporosis. Methods A rat model of osteoporosis was established by bilateral ovariectomy. The rats were randomly divided into sham operation group, model control group, positive control group (alendronate sodium, 1 mg / kg · bw), PP- AKP low and high dose group (400,800 mg / kg · bw). Continuous gavage 90d, 1 / d. At the end of the experiment, the serum levels of tartrate-resistant acid phosphatase, cathepsin K, serum TNF-α and other inflammatory cytokines were detected. The contents of calcium, phosphorus and deoxypyridine in the urine were detected. Results PP-AKP could significantly inhibit the activity of tartrate-resistant acid phosphatase and cathepsin K (P <0.01), and significantly decrease the content of calcium, phosphorus and deoxypyridazine in urine (P <0.01) Necrosis factor, interleukin 6, interleukin 1β, nitric oxide (P <0.01) and cyclooxygenase 2 activity (P <0.01). Conclusion PP-AKP can significantly inhibit bone resorption in rats with osteoporosis. Its mechanism may be related to the inhibition of the secretion of inflammatory cytokines.