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目的 通过调查严重急性呼吸综合征(SARS)患者血清中特异IgG、IgM抗体 ,找出冠状病毒与SARS之间可能存在的病因关糸 ;比较急性期和恢复期血清抗体效价 ,寻求该病毒特有的血清学反应及其临床意义。方法 用新分离SARS病毒作为抗原 ,采用间接免疫荧光 (IFA)和酶联免疫吸附试验 (ELISA)法 ,对广州 4所医院SARS病人的血清标本进行检测。结果 检测临床诊断SARS病人13 0例 ,其中 117例出现病毒特异性抗体 ,阳性率达 90 % ,病人血清IgG抗体滴度 10天后明显上升 ,15天后抗体达到高峰 ,IgM抗体滴度2 0～ 3 0天达到高峰。检测 119例健康接触者和同一流行区 10 0例健康人 ,结果全部为阴性。结论 SARS病人血清与新分离SARS病毒抗原有高水平的特异反应 ,证实病人急性感染了这种新的病毒 ;病人恢复期血清IgG抗体在体内滴度高、持续时间长 ,提示可能是人群保护性抗体
Objective To investigate the possible etiopathogenisis between coronavirus and SARS by investigating the serum specific IgG and IgM antibodies in patients with severe acute respiratory syndrome (SARS). To compare the potency of serum antibodies in acute and convalescent sera, Serological reaction and its clinical significance. Methods Serum samples of SARS patients from 4 hospitals in Guangzhou were detected by indirect immunofluorescence (IFA) and enzyme-linked immunosorbent assay (ELISA) using newly isolated SARS virus as antigen. Results Thirteen cases of clinically diagnosed SARS patients were detected, of which 117 cases showed virus-specific antibodies with a positive rate of 90%. Serum IgG antibody titers increased significantly after 10 days and reached peak after 15 days. IgM antibody titers ranged from 20 to 3 0 days to reach the peak. 119 healthy controls and 100 healthy people in the same endemic area were tested, all of which were negative. Conclusion Serum of patients with SARS has a high level of specific reaction with the newly isolated SARS virus antigens, confirming the acute infection of this new virus. The serum titer and duration of serum IgG in convalescent patients is high, suggesting that it may be protective for the population antibody