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用普通狨猴对甲肝减毒活疫苗（H2株）接种者的粪便中排出的甲肝病毒（HAV）进行毒力／减毒水平检测，并对疫苗病毒及分离出毒株的P1和P2连接区的核苷酸片段进行序列分析。结果表明，4份疫苗病毒经人体增殖后均无毒力回升迹象，分离毒株Ⅱ、Ⅴ与K7疫苗病毒的同源性为99．7％～100％，所编码的114个氨基酸其序列完全相同，属同一亚基因型IB。提示我国至少有2个亚基因型的HAV，值得进一步展开甲肝分子流行病学研究。
The virulence / attenuated level of hepatitis A virus (HAV) excreted in the droppings of live attenuated hepatitis A vaccine (H2 strain) was tested using common marmosets, and the P1 and P2 junction regions of the vaccine virus and isolates were tested Nucleotide sequence of the nucleotide sequence analysis. The results showed that all the four vaccine viruses showed no signs of recovery after being multiplied. The homologies between strains Ⅱ and Ⅴ and K7 vaccine virus were 99.7% ~ 100%. The encoded sequence of 114 amino acids was completely Same, belong to the same subgenotype IB. It is suggested that there are at least 2 subtypes of HAV in our country and it is worth to further investigate molecular epidemiology of hepatitis A.